Abstract

The temperature dependence of the secondary structure of photosynthetic reaction centres from Rhodobacter sphaeroides in solution and in Langmuir-Blodgett film was studied by circular dichroism. It was shown that the secondary structure of the protein was not affected in Langmuir-Blodgett films by heating up to 200°C, while in solution it was completely lost at 55°C. Molecular order rather than decreased hydration degree was held responsible.

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