Abstract
Thermal properties of NAD malate dehydrogenase (MDH) and glutamate oxaloacetate transaminase (GOT) were investigated in two genotypes of Arabidopsis thaliana collected in two sites of contrasting climates. Plants from each genotype were acclimated under controlled conditions at four different thermoperiods: 5–10°C, 7–15°C, 15–25°C and 25–28°C (night/day; photoperiod 16 h). The mitochondrial fraction of MDH of the warm adapted genotype was consistently more thermostable than that of the cold-adapted genotype. No differences in the number of isozymes, consisting of two mitochondrial and four cytosol isozymes, or in their electrophoretic mobility in polyacrylamide gels, were found between the genotypes. MDH preparations from plants acclimated to the coldest thermoperiod were the most thermostable but this phenomenon was not associated with increases in the concentration of reducing sugars, proteins or proline. No differences in the thermostability of GOT were found between plants of the two genotypes acclimated at any of the four thermoperiods. Electrophoretic analyses showed identical GOT profiles for both genotypes consisting of one mitochondrial and one cytosol isozyme which were thermodenatured at the same rate.
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