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Abstract
We have performed thermal melting studies toward developing an empirical formula for PNA-DNA duplexes. Our results indicate the identity and sequence of the junction between the phosphodiester and 2-aminoethylglycine backbones are critical to the overall stability of the duplex and an important design consideration. Thermal melting studies toward developing an empirical formula for PNA-DNA duplexes were performed. The results indicate the identity and sequence of the junction between the phosphodiester and 2-aminoethylglycine backbones is critical to the overall stability of the duplex.
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