Abstract
The inactivation of oxide reductases in mate leaves, a process known in Brazil as sapeco, is carried out at temperatures of 400 to 620°C. High temperatures can produce pyrolysis compounds, such as polycyclic aromatic hydrocarbons. In this study, the time/temperature binomial was determined for the inactivation of peroxidase (POD) in mate in a conveyor belt oven. POD showed greater thermal resistance than polyphenoloxidase. A 22 full factorial design (levels +1 and – 1), with two axial points (levels –α and +α) and three central points (level zero), was used. The biphasic and consecutive step models showed the best fit to the data obtained for the inactivation of POD (R2 = 0.99). The best inactivation was obtained after treatment at 255°C for 20 to 24 s, achieving a green colour the same as that obtained through processing using the traditional procedure.
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