Abstract
1. 1. A procedure for the determination of heat denaturation of hemocyanin by means of changes in specific refractive increment has been presented. The method possesses the advantage of requiring a small volume (0.1 ml) of solution for analysis. 2. 2. Denaturation was found to follow first order kinetics and yielded an activation energy of 80,960 cal mol −1. 3. 3. The rate of denaturation of hemocyanin at constant temperature varied inversely as the first power of the hydrogen ion concentration, suggesting a dissociation of one proton in the range of pH studied. 4. 4. Thermodynamic analogues based upon the theory of absolute reaction rates were calculated from the data. Approximate values of ΔF ∗, ΔH ∗, and ΔS ∗ were found to be approximately 20,700 cal mol −1, 80,000 cal mol −1 and 175 cal degree −1, respectively.
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