Thermal adaptation activates HSP70 synthesis, inhibits overproduction of nitric oxide, and protects the body from acute hypotension during heat shock

Abstract

The role of HSP70 and nitric oxide in antihypotensive effects of thermal adaptation was studied. Western blot analysis and electron paramagnetic resonance were used to determine the contents of HSP70 and nitric oxide. Protective effect of adaptation was evaluated by the limitation of blood pressure drop after heat shock. The formation of protective effects, accumulation of HSP70, and development of the ability to decrease nitric oxide overproduction had similar dynamic patterns and appeared at the same period. Quercetin, an inhibitor of HSP70 synthesis, prevented the development of protective effects. The data suggest that HSP70 accumulated during adaptation prevents heat shock-induced hypotension by restricting NO over-production and interfering with its cytotoxic effects.