Therapeutic Study of Thermosensitive Hydrogel Loaded with Super-Activated Platelet Lysate Combined with Core Decompression Technology for the Treatment of Femoral Head Necrosis.

Zhipeng Huang,Jun Lang,Yinsheng Fu,Wantao Wang,Wenbo Wang,Zhe Zhao

doi:10.1155/2021/7951616

Abstract

Super activated platelet lysate (sPL) is a derivative of platelet-rich plasma (PRP) that contains high levels of several growth factors. In this study, we synthesized a temperature-sensitive hydrogel that contained temperature-sensitive Poly(DL-lactide-glycolide-glycolide acid) (PLGA), SrCl2, and HA, and loaded it with different concentrations of sPL. The hydrogel showed satisfactory encapsulation efficiency and release of the growth factors in a sustained manner, indicating its suitability as a drug carrier. The sPL-loaded hydrogel was inserted into the necrotic femoral head of a rat model and core decompression was applied and resulted in significantly accelerated bone repair and regeneration. Therefore, encapsulation of sPL in a hydrogel scaffolding may be an effective strategy for treating femoral head necrosis.

Highlights

Femoral head necrosis (ONFH) is characterized by osteocytic necrosis and bone marrow necrosis as a result of insufficient or a complete lack of blood supply to the subchondral bone
Temperature-sensitive Poly(DL-lactide-glycolideglycolide acid) (PLGA)/HA/SrCl2 hydrogel loaded with Super activated platelet lysate (sPL) was used to reconstruct the degenerated bone tissue in combination with Core decompression (CD) surgery. sPL was slowly released from the hydrogel in a temperature-sensitive manner and resulted in accelerated bone repair after core decompression of the femoral head and ONFH
The temperature-sensitive PLGA\SrCl2 and HA were mixed at a ratio of 94 : 5 : 1, and the mixture was added to 0, 250, and 500 μl of sPL placed in a magnetic stirrer

Summary

Introduction

Femoral head necrosis (ONFH) is characterized by osteocytic necrosis and bone marrow necrosis as a result of insufficient or a complete lack of blood supply to the subchondral bone. Core decompression (CD) is a joint-preserving surgery [3] that is suitable for the early stage ONFH patients with an intact joint surface [4]. It can reduce intramedullary pressure on the femoral head, accelerate bone regeneration that may form a cavity after core decompression, and reverse femoral head necrosis [5], thereby delaying the progression of the disease and preventing femoral head collapse. Temperature-sensitive PLGA/HA/SrCl2 hydrogel loaded with sPL was used to reconstruct the degenerated bone tissue in combination with CD surgery. SPL was slowly released from the hydrogel in a temperature-sensitive manner and resulted in accelerated bone repair after core decompression of the femoral head and ONFH. Our study has laid the foundation for the clinical application of heat-sensitive hydrogel materials, and for a considerable reduction in the cost of treating ONFH patients

Materials and Methods

In Vivo Experiments

Results and Discussion

Conclusion