Therapeutic potential of root and stem bark of wild medicinal plant Ziziphus mauritiana (Lamk.) against silica induced toxicity in Wistar albino rats

Abstract

Ethnopharmacological relevanceZiziphus mauritiana (Lamk.) of the family Rhamnaceae is a traditional herbal medicinal plant commonly called as ‘ber’ (Indian jujube). It is traditionally used as food source and helps in treatment of various diseases like malaria, asthma, diarrhoea, typhoid, diabetes, skin diseases and acts as a pain killer. Aim of the studyThis study aimed to evaluate the ability of extracts of barks of root and stem of Ziziphus mauritiana (ZM) to cure silica induced toxicity in Wistar albino rats. Materials and methodsExperiments carried out on the acute toxicity studies of silica and therapeutic potential of ZM extracts were based on OECD guidelines and from these results lethal dose (LD50) values were determined. Silica was administered by IP injection at 20 mg/kg b.w. for 10 days and roots and stem barks extracts of ZM were fed orally by gavage at 400 mg/kg b.w. for 21 days. Assessments of biochemical, haematological parameters as well as liver histological examination were performed in the study. ResultsAfter 21 days of oral feeding of extracts of root and stem of ZM, it was found to alter the liver histology. Significant decrease in enzymes such as ALT, AST, ALP, LDH and urea, creatinine and uric acid levels in serum were recorded. Treatment with extracts could help enzymatic activity of liver antioxidant enzymes to settle towards normal level and significant decrease in the lipid peroxidation along with increase in the value of GSH in liver, was also observed. In addition, extracts of ZM also helped to reduce the serum NO, IL-6 and TNF-α level. ConclusionResults of this study has suggested that the extracts of root and stem bark of ZM can be used for therapeutic purpose to prevent silica induced toxicity. ZM extracts could be utilised as natural antioxidant and immunotherapeutic medicines to protect silica induced cell damage.