THERAPEUTIC POTENTIAL OF Citrus aurantium ON UROLITHIASIS INDUCED BY ETHYLENE GLYCOL IN MALE RABBITS

Abstract

Nephrolithiasis is a complex physicochemical event which starts with urinary supersaturation and then leads to aggregation of renal crystal forming constituents within the kidneys.Researches gives much attention for better medical therapy and assess a satisfactory drug to prevent renal stone formation. The purpose of the study was to detect the effectiveness of Citrus aurantium (bitter orange) juice on ethylene glycol induced nephrolithiasis in male rabbits. Animals were divided into five groups (n=8) in which group 1 as control (C), group 2 as ethylene glycol non treated group (EG), group 3 as ethylene glycol group treated with bitter orange juice (EG+B.O), group 4 as ethylene glycol group treated with Citraforte (EG+C) and group 5 as ethylene glycol group treated with bitter orange juiceand Citraforte (EG+B.O+C).Ethylene glycol was givento group 2-5 in drinking water for 21st days for the induction of kidney stones then the treatments were continued for successive 3 weeks. The obtained results showed a significant increase(p≤0.05) in the serum level of total protein, albumin, phosphorus, uric acid, creatinine, blood urea nitrogen BUN,Malondialdehyde (MDA) and a significant decrease in calcium, catalase (CAT), Superoxidase dismutase(SOD) and glutathione (GSH) in the group of rabbits treated with ethylene glycol.All the estimated parameters showed an improvement in their values in the treated groups especially in group 5 which treated with bitter orange juice plus Citra forte. There was a detectable pathological lesion in the kidney tissues of rabbits in ethylene glycol group which improved with treatment by bitter orange juice and or Citraforte. In conclusion:Co-administration of bitter orange (Citrus aurantium) juice with Citraforte initiated a synergistic action for curing urolithiasis induced by ethylene glycol in male rabbits.