Abstract
Objective: The purpose of this research was, synthesis of copper nanoparticles using environment friendly cementation method and evaluate their wound healing property on full-thickness excisional wound.
 Methods: Present study reports the synthesis of CNPs by single-step cementation method. Evaluation of CNPs was endorsed by morphological and chemical properties. Furthermore, CNPs was evaluated for its antibacterial potential and invitro hemocompatibility. Additionally, pharmacological evaluation of CNPs was assessed against excisional wound.
 Results: Characterization of final product indicate, particle size of CNPs were ranging from 100-150 nm. CNPs showed significant antibacterial activity (A= 2.1±0.1 mm, B =2.1±0.1 mm, C = 1.9±0.2 mm, at 10µg/ml), along with superior hemocompatibility (RBC cell survival 97±1 %). Further CNPs formulation shows increased level of anti-inflammatory cytokinin’s (IL-10, 42.7%) as compared to standard (STD), vehicle control, and normal control groups, attributed to accelerated wound healing (p<0.05 vs STD).
 Conclusion: The consequences the present investigation endorse the accelerated wound healing potential of CNPs with its anti-inflammatory potential.
Highlights
Wound healing is a complex biological progression; wounding leads to tissue inflammation and the release of several pro-inflammatory cytokines including interferon-gamma (IFN-γ), interleukin [1] (IL-1), and tumor necrosis factors (TNF)
Atomic absorption spectroscopy (AAS) indicated the decrease in the concentration of Cu2+ions, which have been reduced to Cu° by elemental iron
Our results showed the synthesis of copper nanoparticles (CNPs) from copper nitrate solution by the novel cementation method, with particle size in the range of 100-150 nm
Summary
Wound healing is a complex biological progression; wounding leads to tissue inflammation and the release of several pro-inflammatory cytokines including interferon-gamma (IFN-γ), interleukin [1] (IL-1), and tumor necrosis factors (TNF). The release of pro-inflammatory cytokines recruits monocytes and macrophages [2]. Over activation of proinflammatory cytokines and obstructed instigation of antiinflammatory mediates delayed the healing rate of the wound [4]. Inhibition of the production of inflammatory cytokines, inflammatory transduction cascades, reduction of oxidative factors, and prevention of the microbial growth at the wound site may delay the healing rate [5]. Copper is traditionally known for its exceptional antibacterial and anti-inflammatory properties [8]. Copper enhances the rate of wound healing by providing optimum antibacterial and anti-inflammatory properties [9]
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