Therapeutic efficiency of probiotic lactobacillus acidophilus, lactobacillus rhamnosus and antibiotic against isolates of pathogenic bacterial from skin wound infections

Abstract

Background: Probiotic refer to harmless live normal flora micro-organisms (M.O), which are mostly strains of Lactobacillus spp., Bifidobacterium spp., and others. These microorganisms (M.O) help the host's health when given in the right dosages. Aims: To identify bacterial species isolated from wound infections, and also to evaluate in-vitro the antimicrobial activity of selected probiotic species and antibiotic against some pathogenic bacteria. Methods: Records of wound swabs collected from 102 patients with high suspicion of wound infections. Bacterial colonies were Gram stained and microscopically examined. Biochemical tests were done to identify pathogen species. The microtitre plate method (MTP) was used to detect biofilm formation by bacteria. The Kirby-Bauer disk diffusion method was used for antibiotic testing. The well diffusion method and agar spot method was used for detection probiotic affectivity to a group of bacteria isolated (totally 38 isolates). Results: Prevalence of wound infections was 81.4% (CI: 60.869). A total of 10 species were isolated from 83 infected wounds. The most common bacterial species detected was Staphylococcus aureus (26.5%), followed by Pseudomonas aeruginosa 14.4%, Proteus mirabilis (12%), Escherichia coli 10.8%, and Enterobacter cloacae (8.4%). Polymicrobial infection was found in 25(30.1%) of the samples and was mainly constituted with two species.