Therapeutic Effects of Cold-Pressed Perilla Oil Mainly Consisting of Linolenic acid, Oleic Acid and Linoleic Acid on UV-Induced Photoaging in NHDF Cells and SKH-1 Hairless Mice.

Hyeon Jun Choi,Bo Ram Song,Bae-Hwan Kim,Chung Yeoul Lee,Su Ji Bae,Yun Ju Choi,Hee Seob Lee,Su Jin Lee,Jeong Eun Gong,Ji Eun Kim,Dae Youn Hwang

doi:10.3390/molecules25040989

Abstract

Positive physiological benefits of several plant oils on the UV-induced photoaging have been reported in some cell lines and model mice, but perilla oil collected from the seeds of Perilla frutescens L. has not been investigated in this context. To study the therapeutic effects of cold-pressed perilla oil (CPO) on UV-induced photoaging in vitro and in vivo, UV-induced cellular damage and cutaneous photoaging were assessed in normal human dermal fibroblasts (NHDFs) and HR-1 hairless mice. CPO contained five major fatty acids including linolenic acid (64.11%), oleic acid (16.34%), linoleic acid (11.87%), palmitic acid (5.06%), and stearic acid (2.48%). UV-induced reductions in NHDF cell viability, ROS production, SOD activity, and G2/M cell cycle arrest were remarkably improved in UV + CPO treated NHDF cells as compared with UV + Vehicle treated controls. Also, UV-induced increases in MMP-1 protein and galactosidase levels were remarkably suppressed by CPO. In UV-radiated hairless mice, topical application of CPO inhibited an increase in wrinkle formation, transepidermal water loss (TEWL), erythema value, hydration and melanin index on dorsal skin of UVB-irradiated hairless mice. CPO was observed to similarly suppress UV-induced increases in epidermal thickness, mast cell numbers, and galactosidase and MMP-3 mRNA levels. These results suggest CPO has therapeutic potential in terms of protecting against skin photoaging by regulating skin morphology, histopathology and oxidative status.

Highlights

Photoaging is defined as premature skin aging due to continuous, long-term exposure to chronicUVA and UVB [1]
Various plant oil and their mixtures have been investigated in terms of their abilities to suppress UV-induced photoaging in skin fibroblast cells and hairless mice
Treated groups, dose-dependency was not observed (Figure 4). These results indicate that the high anti-oxidant activity of cold-pressed perilla oil (CPO) was probably associated with its inhibitory effects on UV-induced cell death

Summary

Introduction

Photoaging is defined as premature skin aging due to continuous, long-term exposure to chronicUVA and UVB [1]. Photoaging is defined as premature skin aging due to continuous, long-term exposure to chronic. Connective tissues are damaged and skins exhibited wrinkle formation, laxity, pigment condensation, and thickening [2]. UVB-induced reactive oxygen species (ROS) production in skin activates MAP kinase signaling pathways and stimulates the Molecules 2020, 25, 989; doi:10.3390/molecules25040989 www.mdpi.com/journal/molecules. Various plant oil and their mixtures have been investigated in terms of their abilities to suppress UV-induced photoaging in skin fibroblast cells and hairless mice. The mixture of primrose oils was found to inhibit UV-induced wrinkle formation via the regulations of MMP, MAP kinase, AP-1 transcription factor, and TGF-β2 [5], and the essential oils of Cnidum officinale makino and Ligusticum chuanxiong hort significantly inhibited UV-induced DNA damage and apoptosis in NIH 3T3 cells [6].

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