Abstract
Aim Fuyang Jiedu Huayu (FYJDHY) granules are a combination of five traditional Chinese medicines with known therapeutic effects against chronic liver failure (CLF). The aim of the present study was to investigate the efficacy of FYJDHY to ameliorate the effects of carbon tetrachloride- (CCl4-) induced CLF in rats and to explore the possible molecular mechanisms underlying its therapeutic efficacy. Methods A model of chronic liver failure was established by intraperitoneal injection of 50% carbon tetrachloride into SD rats for 8 weeks. After establishing the model, rats were treated with either low-dose (4.725 kg/d), medium-dose (9.45 kg/d), or high-dose (18.9 g/kg/d) FYJDHY for 2 weeks. After treatment, samples of liver tissue and blood were harvested from rats in each group. Serum ALT, AST, and TBIL levels and prothrombin time were measured using a biochemical analyzer. The expression of Gab1 (Grb2-associated binder 1), TPO (thrombopoietin), and its receptor c-Mpl were measured using quantitative real-time PCR (RT-PCR) and Western blot analysis, and assessment of histological improvement in liver tissue was by H&E-stained tissue sections. Results Compared with the model group, serum ALT, AST, and TBIL levels and PT of rats in the intervention group were significantly reduced (P < 0.05). In addition, FYJDHY alleviated pathological damage to liver tissue and increased the expression of Gab1, TPO, and its receptor c-Mpl in liver tissue, to levels statistically significant compared with the model group (P < 0.05). Conclusions The therapeutic effect of FYJDHY on CLF may be related to the promotion of angiogenesis and improvement in hemopoietic function in individuals suffering from CLF.
Highlights
Chronic liver failure (CLF) is a complication of liver cirrhosis, characterized by a progressive decline in liver function and functional decompensation
Hepatocyte necrosis and apoptosis directly lead to the collapse of liver tissue structure, which is the principal manifestation of chronic liver failure
Fuyang Jiedu Huayu granules (FYJDHY) (15 g of monkshood, 15 g of ginseng, 30 g of red peony, 15 g of rhubarb, and 30 g of wormwood) comprise the refined prescription formed from the new theory of Du Xie–Du Zhuo of traditional Chinese medicine of liver failure, which embodies the therapeutic concept of Jiedu, Huazhuo, and Fuyang for the prevention and treatment of chronic liver failure
Summary
Chronic liver failure (CLF) is a complication of liver cirrhosis, characterized by a progressive decline in liver function and functional decompensation. As a comprehensive medical practice characterized by holistic treatment philosophy, traditional Chinese medicine positively affects many diseases and has successfully helped Chinese individuals for more than 3000 years. In recent years, it has played an important role in the treatment of many types of liver disease, including liver failure. Fuyang Jiedu Huayu granules (FYJDHY) (15 g of monkshood, 15 g of ginseng, 30 g of red peony, 15 g of rhubarb, and 30 g of wormwood) comprise the refined prescription formed from the new theory of Du Xie–Du Zhuo of traditional Chinese medicine of liver failure, which embodies the therapeutic concept of Jiedu, Huazhuo, and Fuyang for the prevention and treatment of chronic liver failure. We constructed a rat model of chronic liver failure and used FYJDHY as an intervention to observe the effect of this prescription on the expression of the connexin Gab (Grb2associated binder 1) [5], TPO (thrombopoietin), and its receptor c-Mpl, the key molecule for the maintenance of hematopoietic stem cell function [6]
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