Abstract
Objective: Hepatic cancer is known as primary liver cancer and hepatocellular carcinoma (HCC). Newly silver nanoparticles gained importance due to its advantages and multiple potential such as molecular imaging agent, antimicrobial, wound healing, anti-inflammatory and anticancer activity. The current study deals to assess therapeutic property silver nanoparticles (AgNPs) against diethylnitrosamine (DENA), and carbon tetrachloride (CCL4) induced hepatic cancer.
 Methods: Thirty male albino rats (200-250g) were distributed into four groups and hepatic cancer was induced with a single intraperitoneal dose of 200 mg/kg body weight of DENA. Two weeks later, animals received subcutaneous injections of CCl4 once a week in a dose of 3 ml/kg body weight for 6weeks. Serum biomarkers, antioxidants enzymes, inflammatory markers were evaluated to find the anti-proliferative potential of silver nanoparticles. Histological evaluation and microscopic reports were also done to document the results of the current work.
 Results: AgNPs significantly recover the serum marker enzymes of hepatic parameter AST, ALT, ALP, and total bilirubin and also decreased the levels of NO, IL-6 and TNF-α. Histopathological features also exhibited recovery of a hepatic architecture in cancer-induced rats. Moreover, the immunohistochemical investigation demonstrated that the levels of PCNA, and Caspase-3, which are hepatocarcinogenic markers, were significantly improved by AgNPs.
 Conclusion: These results concluded that AgNPs showed promising curing effects on hepatocellular ailments.
Highlights
The liver is the largest solid organ and one of the most vital organs that functions as xenobiotic and drug, energy, protein and amino acid metabolism, storage of glucose in the form of glycogen, storage of vitamins, and regulation of hormonal functions
Values are presented as mean±standard error of the mean (SEM)*: statistically significant compared to corresponding value Normal control (NC) group (P
Based on the present study results, we suggest the therapeutic effect of AgNPs synthesized by the green method using Nigella sativa against diethylnitrosamine and carbon tetrachlorideinduced hepatocellular carcinoma in rats
Summary
The liver is the largest solid organ and one of the most vital organs that functions as xenobiotic and drug, energy, protein and amino acid metabolism, storage of glucose in the form of glycogen, storage of vitamins, and regulation of hormonal functions. Ozougwu stated that a total loss of liver function might lead to death within minutes, indicating the liver‘s great importance. Hepatocellular carcinoma (HCC) is a life-threatening neoplasm that originated from hepatocytes, representing approximately 80% of liver cancer and 3rd most common cancer-related death [1]. Human cancers are related to chronic inflammation caused by chemical, biological and, physical factors. The effect of inflammation-fibrosis-carcinoma axis acts as a bridge from inflammation to cancer, and promotes inflamed liver development to fibrosis/cirrhosis and HCC [3]. Upon exposure to risk factors like alcohol, viruses, parasites and, toxic substances, the hepatic injury resulted in the degeneration and inflammation, leading to chronic liver diseases, which may further progress to different stages of fibrosis, cirrhosis, and HCC. HCC is the final stage of this process [3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
