Therapeutic drug monitoring of vancomycin and voriconazole by liquid chromatography-tandem mass spectrometric method

Abstract

A reliable liquid chromatography-tandem mass spectrometric(LC-MS/MS) method was developed and validated for the simultaneous determination of vancomycin and voriconazole in human plasma. The analytes and internal standard 10-hydroxycarbazepine were separated at a flow rate of 0.9 mL/min using a Zorbax SB-C18 column (50 mm×4.6 mm, 2.7 μm). Positive ion electrospray ionization was used to detect vancomycin, voriconazole and intrenal standard(IS) 10-hydroxycarbazepine followed by multiple reaction monitoring(MRM) of the transition at m/z 725.5→144.2, 350.3→281.0 and 253.1→208.0, respectively. The total run time for both vancomycin and voriconazole samples was 5 min; 0.30 μg/mL was the lower limit of quantification. The precision of intraday and interday was no more than 12.4%. The method was successfully and resoundingly applied in therapeutic drug monitoring of 156 patients treated with vancomycin and voriconazole.