Abstract
Nicotinamide (NAM) is a group B3 vitamin involved in a wide range of biological processes. Recently, the anti-inflammatory properties of NAM have been revealed. In this study, we investigated the therapeutic effects of NAM in murine models of endotoxemia and sepsis. Endotoxemic liver injury was induced by intraperitoneal injection of lipopolysaccharide (LPS) into d-galactosamine (d-Gal)-sensitized mice. Lethal endotoxemia was induced by intraperitoneal administration of LPS at a dose of 20mg/kg. Polymicrobial sepsis was induced by cecal ligation and puncture (CLP). In mice challenged with LPS/d-Gal, treatment with NAM significantly deceased serum aminotransferases level and alleviated hepatic lesions. NAM also reduced serum tumor necrosis factor-α level and attenuated apoptosis in liver, as assessed by terminal deoxynucleotidyl transferase-mediated nucleotide nick end labeling (TUNEL) staining and measurements of caspases activities. Survival analysis indicated that NAM reduced the mortality rate of LPS/d-Gal-challenged mice. In mice with lethal endotoxemia, NAM reduced serum level of pro-inflammatory cytokines and multiple organ damage as evidence by improved morphological lesion, reduced lung wet to dry ratio as well as decreased serum level of aminotransferase and blood urea nitrogen. In survival analysis, treatment with NAM increased the survival rate of mice with lethal endotoxemia or CLP-induced polymicrobial sepsis. Taken together, treatment with NAM might provide therapeutic benefits in sepsis, which attenuated inflammatory injury and improved the survival rate.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
