THERAPEUTIC ACTIVITY OF POLYHERBAL FORMULATION AGAINST PASSIVE ANAPHYLAXIS BY USING RAT MESENTERIC MAST CELLS

Abstract

Anaphylaxis is the result of alteration in mast cell physiology and is responsible for the various physiological changes which are observed during anaphylaxis. The symptoms of anaphylaxis are due to the release of the histamine from the mast cells and it results mainly due to the degranulation of the mast cells. Antianaphylactic activity of polyherbal formulation against passive anaphylaxis was evaluated with the help of sheep serum induced passive anaphylaxis model and passive paw anaphylaxis model. Sheep serum induced passive anaphylaxis model was carried out by wistar rats. The selected rats were administered intraperitoneally with serum of active anaphylactic rats. After 48 h all the rats are administered intraperitoneally with 1ml of sheep serum. The rats which are pre-treated with prednisolone (10 mg), polyherbal formulation (250 mg) were analyzed for the mast cell degranulation. Passive paw anaphylaxis model was carried out by subcutaneous administration of 0.1 ml serum of active anaphylactic rats into the plantar region of left hind paw of rats. After 24 h the left hind paw of rats was administered subcutaneously with 0.1 ml of egg albumin. The rats which are pre-treated with prednisolone (10 mg), polyherbal formulation (250 mg) were analyzed for the increase in paw volume. Antianaphylactic activity of polyherbal formulation on the mesenteric mast cells of the rat may be possibly due to the membrane stabilizing potential.