Abstract
1. Dna Extraction from Vegetative Tissue for Next Generation Sequencing Agnelo Furtado 2. DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples) Chiaki Mutou, Katsunori Tanaka, Ryuji Ishikawa 3. RNA Extraction from Cereal Vegetative Tissue Julie A. Pattemore 4. RNA Extraction from Developing or Mature Wheat Seeds Agnelo Furtado 5. cDNA Library Preparation Maarten Kooiker and Gang-Ping Xue 6. Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants Siddanagouda S. Biradar, Xiaojun Nie, Kewei Feng and Song Weining 7. The Polymerase Chain Reaction (PCR) - General Methods Daniel L. E. Waters and Frances M. Shapter 8. Mutation and Mutation Screening L. Slade Lee, Bradley J. Till, Helen Hill, Owen A. Huynh, and Joanna Jankowicz-Cieslak 9. The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression Timothy L. Fitzgerald and Richard B. McQualter 10. Cloning of DNA Fragments: Ligation Reactions in Agarose Gel Agnelo Furtado 11. Rapid Cloning of Genes and Promoters for Functional Analyses Peer M. Schenk 12. Genome Walking Frances M. Shapter and Daniel L.E. Waters 13. Functional Analysis by Protein Biochemistry Louis M. T. Bradbury 14. Genomic Southern Blot Analysis Leigh Gebbie 15. Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs Shazia Iram 16. Protein Blotting-Protocol for Beginners Lars A. Petrasovits 17. Genetic Transformation of Wheat via Particle Bombardment Caroline A. Sparks & Huw D. Jones 18. Sorghum Genetic Transformation by Particle Bombardment Guoquan Liu, Bradley C. Campbell and Ian D. Godwin 19. Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery Caroline A. Sparks, Angela Doherty & Huw D. Jones 20. A Protocol for High-throughput Agrobacterium-mediated Barley Transformation Wendy A Harwood 21. Agrobacterium-mediated Transformation- Rice Transformation Inez H. Slamet-Loedin, Prabhjit Chadha-Mohanty, Lina Torrizo 22. Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos Hyeyoung Lee and Zhanyuan J. Zhang 23. A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops Zining Wang 24. A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data Gopala Krishnan, S., Daniel L. E. Waters and Robert J. Henry
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