Theranostic applications of phage display to control leishmaniasis: selection of biomarkers for serodiagnostics, vaccination, and immunotherapy.

Eduardo Antonio Ferraz Coelho,Carlos Alberto Pereira Tavares,Lourena Emanuele Costa,Luiz Ricardo Goulart,Manuel Soto,Miguel Angel Chávez-Fumagalli

doi:10.1590/0037-8682-0096-2015

Abstract

Phage display is a high-throughput subtractive proteomic technology used for the generation and screening of large peptide and antibody libraries. It is based on the selection of phage-fused surface-exposed peptides that recognize specific ligands and demonstrate desired functionality for diagnostic and therapeutic purposes. Phage display has provided unmatched tools for controlling viral, bacterial, fungal, and parasitic infections, and allowed identification of new therapeutic targets to treat cancer, metabolic diseases, and other chronic conditions. This review presents recent advancements in serodiagnostics and prevention of leishmaniasis -an important tropical parasitic disease- achieved using phage display for the identification of novel antigens with improved sensitivity and specificity. Our focus is on theranostics of visceral leishmaniasis with the aim to develop biomarker candidates exhibiting both diagnostic and therapeutic potential to fight this important, yet neglected, tropical disease.

Highlights

Leishmaniasis is a group of cutaneous and visceral infections caused by protozoan parasites belonging to the genus Leishmania(1)
This review presents recent advancements in serodiagnostics and prevention of leishmaniasis -an important tropical parasitic disease- achieved using phage display for the identification of novel antigens with improved sensitivity and specificity
This review explores potential use of phage display technology for theranostics of leishmaniasis, focusing on recent improvements in biomarker discovery strategies that have led to the identification of novel vaccine candidates and diagnostic markers for visceral leishmaniasis (VL)

Summary

Introduction

Leishmaniasis is a group of cutaneous and visceral infections caused by protozoan parasites belonging to the genus Leishmania(1). This review presents recent advancements in serodiagnostics and prevention of leishmaniasis -an important tropical parasitic disease- achieved using phage display for the identification of novel antigens with improved sensitivity and specificity.

Results

Conclusion