Abstract

Thepuf operon ofRhodobacter capsulatus contains genes that encode structural proteins found in photosynthetic pigment-protein complexes, as well as the recently discoveredpufQ gene. We have investigated the function of thepufQ gene product and found that it is essential for the formation of mature B800-850 light-harvesting complexes. However, we have also shown thatpufQ does not seem to affect transcription, mRNA accumulation, or translation of the B800-850 complex structural genes. Therefore, the role of thepufQ gene product is posttranslational, and it may function in assembly of pigments and proteins into photochemically active B800-850 complexes, as well as in bacteriochlorophylla biosynthesis (2).

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