Abstract
Abstract Electrical diffuse layers established by arrangement of ions along a metal capillary column can be used as the virtual “stationary phase” for charged macromolecular separations and determinations. Preliminary studies have indicated that resolution depends on solvent flow rate, pH, column diameter, etc., as well as the applied potential. In this communication we report a theory which underlies the mechanism of retention of charged macro-molecules, e.g., proteins, on the column. The utility and difficulties of this novel fractionation technique will also be discussed.
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