Abstract
A spherical focal spot can be generated by focusing radially polarized laser beams in a 4Pi focusing system, which will result in an increase in axial resolution in microscopy or higher axial trapping efficiency. We demonstrate the possibility of continuous shifting of the spherical focal spot along the prescribed trajectory in three-dimensional space by suitably modulating the phase of the input fields at the pupil planes of the microscope objective lenses. This may provide an alternative to moving trapped particles or scanning a specimen without moving either laser beams or the stage.
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