Theoretical Aspects of the Resolution of Lipid Molecular Species Containing Unsaturated Fatty Acid on Reverse Phase High Performance Liquid Chromatography

Abstract

In this study, attention was directed to the theoretical aspects of the resolution of lipid molecular species containing unsaturated fatty acids on reverse phase high performance liquid chromatography (HPLC).The chemical potential of a lipid molecule in the chromatographic system was influenced by the sum of dipole, induction, and dispersion effects of van der Waals forces, among lipid molecules, mobils phase, and stationary phase.Even in reverse phase HPLC, dipole effect was concluded to exert the greatest effect. Thus when polar domains such as double bonds are situated far away from each other, dipole moment may be large compared to that when they are near each other. Accordingly, the former which has greater affinity toward the polar mobile phase should elute earlier than the latter.Analytical data on relative retention time of molelcular species of diacyl glycerolipid on reverse phase HPLC confirmed this to actually the case.