Theoretical and Experimental Research on Multi-Layer Vessel-like Structure Printing Based on 3D Bio-Printing Technology.

Huanbao Liu,Xuewei Li,Ruichun Dong,Xiang Cheng,Guangxi Zhao,Guangming Zheng,Xianhai Yang

doi:10.3390/mi12121517

Abstract

Cardiovascular disease is the leading cause of death worldwide. Traditional autologous transplantation has become a severe issue due to insufficient donors. Artificial blood vessel is an effective method for the treatment of major vascular diseases, such as heart and peripheral blood vessel diseases. However, the traditional single-material printing technology has been unable to meet the users’ demand for product functional complexity, which is not only reflected in the field of industrial manufacturing, but also in the field of functional vessel-like structure regeneration. In order to achieve the printing and forming of multi-layer vessel-like structures, this paper carries out theoretical and experimental research on the printing and forming of a multi-layer vessel-like structure based on multi-material 3D bioprinting technology. Firstly, theoretical analysis has been explored to research the relationship among the different parameters in the process of vessel forming, and further confirm the synchronous relationship among the extrusion rate of material, the tangential speed of the rotating rod, and the movement speed of the platform. Secondly, sodium alginate and gelatin have been used as the experimental materials to manufacture the vessel-like structure, and the corrected parameter of the theoretical analysis is further verified. Finally, the cell-loaded materials have been printed and analyzed, and cell viability is more than 90%, which provides support for the research of multi-layer vessel-like structure printing.

Highlights

Traditional 3D printing technology has adopted the stacking printing molding method to manufacture vessel-like structures, which has the characteristics of a wide printing space without hindrance
3D bioprinting technologies have been applied to the fabrication of a vessel-like structure embedded with cells
We can draw conclusions that: (a) both theory and the experiment proved that the material extrusion rate, the rotating shaft tangential velocity, and the platform movement speed have an obvious synchronous relationship, 4

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. According to relevant reports [1], the organ donation rate in China has reached. 2/million, and the shortage of organ transplant donors is serious. With the development of or manufacturing technology [4,5,6] is an important means of interventional therapy for vascular diseases, which has a broad application prospect in the treatment of vascular diseases due to its advantages of biological adaptability and immunity. Due to the diversity of vascular structures, traditional single-material printing technology [7] has been unable to satisfy the demand of vessel-like printing. An effective printing method is urgently needed to realize the forming and manufacturing of multi-layer vessel-like structures [8]

Methods

Results

Conclusion