Abstract
Fresnel Incoherent Correlation Holography (FINCH) enables holograms to be recorded from incoherent light with just a digital camera and spatial light modulator. We previously described its application to general three dimensional incoherent imaging and specifically to fluorescence microscopy, wherein one complex hologram contains the three dimensional information in the field of view, obviating the need for scanning or serial sectioning. We have now further analyzed FINCH in view of linear system theory and in comparison to conventional coherent and incoherent two dimensional imaging systems. We demonstrate, theoretically and experimentally, improved resolution by FINCH, when compared to conventional imaging.
Highlights
Digital coherent holography has unique advantages for many imaging applications
In the present study we extend our investigation of Fresnel Incoherent Correlation Holography (FINCH), a way to utilize holography with incoherent light, and which is another example of using digital holography in microscopy
In the experiments presented here, the identical smallest features on the highest resolution USAF chart were imaged at the plane of focus by three methods and compared; 1) conventional high resolution fluorescence microscopy with all glass optics including a matched and properly configured microscope tube lens, 2) microscopy which utilized the spatial light modulator (SLM) as a tube lens to focus the image upon the camera and 3) holograms captured with FINCH and reconstructed at the best plane of focus
Summary
Digital coherent holography has unique advantages for many imaging applications. In some applications the recorded holograms contain three dimensional (3D) information of the observed scene [1], in others the holograms are capable of imaging phase objects [2,3]. Holography enables implementing super resolution techniques [4] and even makes it possible to image objects covered by a scattering medium [5]. Because of these advantages, digital holography has become important in optical microscopy. Examples of utilizing digital holography as the basis for optical microscopes are the recently published studies of lensless compact holography-based microscopes [6,7,8]. Another example of using digital holography in microscopy is the holographic coherent anti-Stokes Raman microscope [9]. In the present study we extend our investigation of Fresnel Incoherent Correlation Holography (FINCH), a way to utilize holography with incoherent light, and which is another example of using digital holography in microscopy
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