Theoretical and experimental analytical studies on potassium clavulanate

Abstract

The spectral and chromatographic procedures (FT-IR, D-UV, TLC, DSC, HPLC-DAD) were developed as alternative tools, to these which are recommended by pharmacopeial guidelines, for identification of potassium clavulanate. As a support for the studies quantum chemical calculations based on the density functional theory (DFT) were used. For a determination of potassium clavulanate in the presence of degradation products two analytical methods were determined: HPLC-DAD and derivative spectroscopy. In the HPLC-DAD method, C-18 stationary phase and 12 mM ammonium acetate-acetonitrile (96:4 V/V) were used. A quantitative determination of potassium clavulanate was carried out by using PDA detector at 220 nm, with a flow rate of 1.0 mL min-1. In derivative spectroscopy the change of values of amplitudes of potassium clavulanate and the first-derivative absorption spectra were used (λ=228 nm) for its determination.