Abstract
A previous study reported that coculturing bovine cumulus-oocyte complexes (COCs) with theca cell monolayers maintained oocytes in meiotic arrest. The present study evaluated whether the protein secretion pattern in this system is different between theca cells and granulosa cells and whether the presence of COCs influences their pattern of secretion. Follicular cells were isolated from 2- to 5-mm follicles and cultured in TCM-199 supplemented with 10% fetal calf serum. Theca cell monolayers maintained COCs but not denuded oocytes (DOs) in meiotic arrest. Monolayers were incubated for 6 hr in medium supplemented with radioactive L-[35S]methionine. The patterns of protein secreted in the medium were analyzed by electrophoresis SDS-PAGE 10%. These results showed that theca cell monolayers secreted two major proteins. This pattern was different from the protein pattern secreted by granulosa cell monolayers. The molecular weights of these proteins were estimated to be 214 and 190 kDa. Coculturing COCs with theca cell monolayers during the labeling revealed that COCs modulated the secretion of theca cell monolayers. When theca cells were grown on collagen-coated wells, the monolayers did not maintain the oocytes at the germinal vesicle (GV) stage. The secretion of the 214-kDa protein also decreased. Then, when theca cell monolayers are effective to maintain oocytes in meiotic arrest, the cells especially secreted the 214-kDa protein. In conclusion, the 214-kDa protein secreted by theca cell monolayers may play a role in the process of maintaining oocytes in meiotic arrest. Mol. Reprod. Dev. 50:200–206, 1998. © 1998 Wiley-Liss, Inc.
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