Abstract
Herridge, D. F., O'Connell, P. and Donnelly, K. 1988. The xylem ureide assay of nitrogen fixation: sampling procedures and sources of error.—J. exp. Bot. 39: 12-22. The relative abundance of ureides [(ureide-N/ureide-N + NOj-N + a amino-N)x 100] in vacuum extracted xylem exudate of soyabean (Glycine max [L.] Merr.) can be used to quantify N2 fixation activity. We report experiments designed to (1) develop appropriate procedures for sampling and storage of xylem exudate extracted from plants in the field and (2) develop a single plant, non-destructive sampling procedure, useful for evaluation of segregating breeding material. Soyabean cultivars Bragg, Essex, Davis, Hardee and Dodds were grown at three sites at Breeza, New South
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