The xanthophyll cycle pigments in Secale cereale leaves under combined Cd and high light stress conditions

Abstract

Leaves of Secale cereale seedlings were exposed to high light illumination (1200 μmol m −2 s −1) and Cd ions at 5 or 50 μM concentrations. Influence of these stress factors on violaxanthin cycle pigments content was analysed chromatographically. Chlorophyll a fluorescence induction was used to analyse response of PSII to stress conditions and contribution of light-harvesting complex (LHCII) in non-photochemical quenching of excitation energy. The Cd-induced all- trans violaxanthin isomerization was analysed by HPLC technique in acetonitrile:methanol:water (72:8:3, v/v) solvent mixture. Interestingly, in the control and Cd-treated leaves subjected to high light, photochemical utilization of absorbed energy increased. This indicates plant adaptation to high light stress. In control plants high light caused zeaxanthin formation, however, the presence of Cd in the nutrient solution resulted in reduction of the second step of violaxanthin de-epoxidation process and anteraxanthin accumulation. In this study we have also shown, that non-photochemical quenching can be independent of anteraxanthin and zeaxanthin content. The particular increase in the cis isomers fraction in Cd-treated leaves has been explained in terms of a direct metal–pigment interaction as confirmed by Cd-induced all- trans violaxanthin isomerization in organic solvent, leading to formation of 13- cis, 9- cis and 15- cis isomers.