The ‘Widow Effect’ and its Consequences for Reproduction in Burying Beetles, Nicrophorus vespilloides (Coleoptera: Silphidae)

Abstract

AbstractBurying beetles tend their young on small vertebrate carcasses, which serve as the sole source of food for the developing larvae. Single females are as proficient at rearing offspring as male‐female pairs, yet males opt to remain with their broods throughout most of the larval development. One potential benefit of a male's extended residency is that it affords him the opportunity of additional copulations with the female, which could ensure his paternity in a replacement brood should the female's first egg clutch fail to hatch. We tested this hypothesis by manipulating males' access to their mates during the production of replacement clutches, using genetic colour markers to determine the paternity of offspring. Females were induced to produce a replacement brood by removing their first clutch of eggs. In one experimental treatment, we removed the female's mate upon the removal of her first egg clutch (‘widowed’ females); in a second treatment, the female was permitted to retain her mate up until she produced a replacement clutch. There was no significant difference in paternity between males removed from females before the initiation of replacement clutches and those permitted to remain with their mates. However, widowed females produced fewer offspring in replacement broods than did females permitted to retain their mates. This difference occurred primarily because a significantly greater proportion of widowed females opted not to produce a replacement clutch, a result we refer to as the ‘widow effect’. This widow effect was further shown in those replicates in which females of both treatments produced replacement clutches: widowed females took significantly longer to produce a replacement clutch than did females permitted to retain their mates. The loss of her mate could be a signal to a female that a take‐over of the carcass is imminent. Her reluctance to produce a replacement clutch under these circumstances might constitute a strategy by which she conserves carrion for a subsequent reproductive attempt with an intruding male successful at ousting her previous mate. Regardless of its functional significance, the widow effect favours the extended residency of males and therefore contributes to the selective maintenance of male parental care.