The Whole genome sequence for Acinetobacter baumannii strain in Iraqi Hospitals Outbreak

Abstract

A study was conducted to identify specialized F plasmid genes and regulatory systems in Acinetobacter baumannii. These genes could be very important for the capacity of these species to coexist with the human host and show a wide range of diversity in genes that contribute to antibiotic resistance. Identification of 50A. baumannii isolates was carried out through morphology and culture on CHROM agar and genotypic identification was performed using blaOxa-51 gene. Out of 50, one AB isolate was chosen for whole genome sequencing (WGS) using Illumina MiSeq technology. The analysis identified specialized genes in these isolates that contribute to antibiotic stress and lipopolysaccharide barrier, including complex sets of partial and complete integrons and transposons. The recent findings, transferring of A. baumannii plasmid genes are inducing under the regulatory system to which the plasmid elements confer Adaptation such as F-plasmid genes transfer in A.baumannii induced by antibiotic stress under the control of transcriptional factor regulation system in order to antimicrobial drug resistance. The selected A. baumannii isolate, IS1- A.baumannii 32 contain five global regulator systems .Our study were provided detailed genomic picture in detected both innate and acquired plasmid-encoded AMR genes. Study aim is the role of genetic regulation to control the transcription, virulence factors and antibiotic resistance related mechanism in A.baumannii .