The warm ischemia time limit of non-heart-beating donors heart for transplantation in Canis familiaris

Abstract

Objective To study the warm ischemia time limit of non-heart-beating donors ( NHBDs) heart for transplantation. Methods The NHBDs heart endured different warm ischemia time limit by asphyxiation or depletion method in Canis familiaris ( 15 ± 3) kg, being harvested and preserved in 4 ℃HTK solution for transplantation. The NHBDs heart endured 0 min warm ischemia time as control. All NHBDs hearts were preserved for 2 h and transplanted to weight-matched recipients. The assist circulation was removed when the donor heart re-beat for 60 min, and the hemodynamic parameters were monitored at 3 h after reperfusion. Meanwhile, cardiac ultramicrostructure was also examined at 3 h after reperfusion. Results The warm ischemia time limit was 16 min in NHBDs heart induced by asphyxiation, and 27 min in NHBDs heart induced by depletion method in Canis Familiaris. The diastolic function of left ventricle ( - dp/dtmax) was decreased significantly in asphyxiation NHBDs heart ( 1361.68 ± 231.47 vs 1641.68 ±192.47 ,P ＜0. 05), but there was no significant difference in contractile function between asphyxiation or depletion Methods (P ＞ 0. 05 ). Conclusion The NHBDs heart harvested by asphyxiation or depletion method in Canis Familiaris all had a warm ischemia time limit and break point of function. Key words: Non-heart-beating donors; Heart transplantation; Warm ischemia time; Canisfamiliaris