Abstract
This research was carried out to examine the effect of various volumes (0.5, 0.75, 1.5 and 2 mL) of the frozen sample on cryopreservation of sturgeon sperm and also the possibility of using the method of vitrification of sperm under deep low-temperature cooling in the form of thin films on nets. The object of the study was the spermatozoa of the Russian sturgeon (Acipenser gueldenstaedtii Brandt, 1833) and the Siberian sturgeon of the Lena population (Acipenser baerii Brandt, 1869). There is a direct relationship between the volume of frozen material and the survival rate of defrosted sperm. With the increase in freeze sample preservation frozen-melted cells is falling, as is the range of cooling rate to freeze the sample, in which the majority of cells are frozen at a speed different from the optimal values. When cryopreservation of a sperm smear in the form of a thin film, the analysis of cell movement activity after defrosting showed the suitability of such sperm for use in the fish-breeding process. The highest life time of the sperm as it was observed during the freezing of the films on the plastic samples.
Highlights
In the context of global environmental problems, the problem of preserving biological diversity has acquired special significance and relevance [1,2,3]
This research was carried out to examine the effect of various volumes (0.5, 0.75, 1.5 and 2 mL) of the frozen sample on cryopreservation of sturgeon sperm and the possibility of using the method of vitrification of sperm under deep low-temperature cooling in the form of thin films on nets
The accumulated data on low-temperature preservation of biological objects to date indicate that the process of long-term storage of biomaterial at low temperature does not significantly affect the preservation of cells after freezing-thawing [8,9,10,11]
Summary
In the context of global environmental problems, the problem of preserving biological diversity has acquired special significance and relevance [1,2,3]. This research was carried out to examine the effect of various volumes (0.5, 0.75, 1.5 and 2 mL) of the frozen sample on cryopreservation of sturgeon sperm and the possibility of using the method of vitrification of sperm under deep low-temperature cooling in the form of thin films on nets.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
