Abstract
ABSTRACT Employing the hyphenated technique, gas chromatography/vapor phase infrared spectrometry/mass spectrometry (GC/VP-IR/MSD), the major volatile and semivolatile constituents of essential oils prepared by supercritical fluid (CO2) and methylene chloride extractions of selected tobaccos are presented. The major volatile and semivolatile components of both the supercritical fluid and methylene chloride extracts are nicotine, neophytadiene and hexadecanoic acid comprising approximately 85% by weight. Acids, ketones, amines and alcohols comprise the balance of the volatile and semivolatile components. All of the components of the extracts (supercritical fluid or methylene chloride) identified in this study have been previously identified in tobacco and/or tobacco smoke. The qualitative nature of these extracts is similar to those previously reported. The supercritical fluid extraction (SFE) approach provides a significant improvement over currently employed techniques such as solvent and Soxhlet extractions. Notable areas of improvement include reductions in lab space requirements, sample process time, material costs, potential solvent residual contamination and manpower requirements.
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