The vitellogenesis-inhibiting hormone (SpVIH) of the crab Scylla paramamosain is not likely to have a vitellogenesis-inhibiting function in the ovary

Abstract

The function and molecular characterization of the previously reported crab (Scylla paramamosain) vitellogenesis inhibiting hormone SpVIH is still unclear. In this experiment, SpVIH’s bioinformatic and functional characterizations were analyzed. Sequence analysis showed SpVIH was clustered with several type II MIH/GIHs of the Brachyura. The expression of SpVIH in the eyestalk was low in the early ovarian developmental stage I and increased with the advancement of ovary maturation. Interestingly, the SpVIH transcript was still at a high level in the ovary of females at the middle to late maturation stage. SpVIH’s function was studied according to the expression of vitellogenin (Vg) and vitellogenin receptor (VgR) in the ovary and hepatopancreas after incubation with SpVIH-dsRNA and recombinant SpVIH protein (rSpVIH). In vitro assay results indicated that rSpVIH has a significant stimulative effect on the expression of Vg in the hepatopancreas in the middle stage of vitellogenesis and in the ovary of each vitellogenesis stage. However, SpVIH-dsRNA has no significant effect on the expression of Vg when SpVIH-dsRNA is introduced to the ovary and hepatopancreas at the early, middle, and late stages of vitellogenesis. Furthermore, the co-incubation experiment result indicated that the eyestalk consists of factor(s) that can significantly up-regulate the expression of Vg in the ovary, with a further combination of dsVIH and eyestalk under incubation, the significant increase of Vg-mRNA expression levels were also found in the ovary of four ovarian development stages of S. paramamosain. Contrary to the previous reports, SpVIH does not possess a vitellogenesis-inhibiting function but can promote vitellogenesis in the ovary and hepatopancreas.