Abstract
Summary A method of determining differences in virulence among avian mycoplasma species and strains was investigated using the chick embryo. Embryos at several stages of development were used. Strains of Mycoplasma galliscpticum (S6) of high and low passage in artificial media and M. gallinarum of high passage were inoculated separately into groups of eggs. Inoculated eggs were incubated at 34, 37 or 40°C and mortality observed. A characteristic and reproducible pattern of embryo death was obtained in which significantly greater mortality occurred with M. galliscpticum (S6) of low passage than with other mycoplasma.
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