The Vicious Cycle of Nonhealing Neointima in Fabric Vascular Prostheses

Abstract

Delayed neointimal healing of a fabric vascular prosthesis was investigated in an animal study focusing on the relationship between red thrombus, fibrinolysis, and endothelialization on the luminal surface. Fabric vascular prostheses were implanted into the descending aortas of 72 dogs. Fifty-nine grafts were explanted from 1 h to 1,705 days after implantation. One hour after implantation, the graft wall was red in color due to fresh thrombus; however, at 1 day the luminal surface became white. Red thrombus reappeared at 1 week and remained present in the long-term. Microscopically the initial red thrombus contained numerous erythrocytes. The white thrombus at 1 day was composed of a dense fibrin network without erythrocytes. At 2 days numerous lacunae appeared in the fibrin layer, and at 3-5 days cavernae and low density fibrin areas were present secondary to fibrinolysis. These areas allowed the blood components to infiltrate into the fibrin layer, and as a result red thrombus reformed within it. The thrombi on the luminal surface in the long-term was always red in color and composed of complicated, multiple stages of thrombus formation, i.e., fresh thrombus with erythrocytes, dense fibrin without erythrocytes, low fibrin density areas, lacunae and cavernae in the fibrin layer, and blood component infiltration into these spaces. Thrombus was always newly formed and present, and involuted in parallel due to fibrinolysis, suggesting that these phenomena perpetuated in a vicious cycle. However, at the anastomoses fibrinolysis was present, but blood component infiltration was prevented by the endothelial cell lining. These results suggest that endothelialization may arrest the vicious cycle of nonhealing neointima in fabric vascular prostheses.