The Viability of anaerobic bacteria from beef cattle feces in liquid media

Abstract

The purpose of this paper is to know the viability of anaerobic bacteria from beef cattle feces in liquid medium incubated at temperature 25oC and 39oC aims to apply as biogas starter. The research was done by the explorative method and the obtained data was analyzed descriptively. The anaerobic bacteria were grown in 98-5 medium and incubated at temperature 25oC and 39oC, observed for 2 months and the analysis was done weekly. The parameters observed were a number of anaerobic bacteria, biogas volume, and percentage of biogas. The results showed that at the beginning of incubation at temperature 25oC, the number of anaerobic bacteria 1,250 x 1010 cfu/mL, after incubating for 2 months, the number of anaerobic bacteria tend to decrease accumulatively reached 230x1010 cfu/mL, the volume of biogas production was 6 mL, the percentages of biogas production were CH4 = 0.0993%, CO2 = 1.1287%, N2, and O2 = 86.163%. At temperature 39oC, the number of anaerobic bacteria, in the beginning, accumulatively reached 585 x 1010 cfu/mL, after incubating for 2 months, the number of anaerobic bacteria decreased accumulatively reached 180x1010 cfu/mL, the volume of biogas production was 4 mL, the percentages of biogas production were CH4 = 0.134%, CO2 = 2.4714%, N2, and O2 = 89.4961%. It was concluded that an anaerobic bacterial of beef cattle feces incubated at temperature 39oC in a liquid medium generated a high survival and turn to be highly potential as a starter of biogas.