The vesicular GABA transporter VGAT is targeted to a distinct set of synaptic vesicles

Abstract

Event Abstract Back to Event The vesicular GABA transporter VGAT is targeted to a distinct set of synaptic vesicles Jean-Luc Boulland1 and Farrukh A. Chaudhry1* 1 University of Oslo, The Biotechnology Centre and Centre for Molecular Biology and Neuroscience, Norway After its exocytotic release, GABA may be translocated into nerve terminals for reuse, or transported to perisynaptic glial processes where it is converted to glutamine which then is transported back to the nerve terminals for regeneration of GABA. We have characterized a family of glutamine transporters (Slc38) and demonstrated their involvement in this GABA-glutamine cycle. We have shown that the system N transporter SN1 is enriched on perisynaptic glial processes where it mediates release of glutamine. Glutamine uptake into neurons is mediated by system A transporters and SAT1 is particularly pronounced in GABAergic neurons. Subsequently the vesicular GABA transporter VGAT mediates accumulation of GABA into synaptic vesicles. Interestingly, VGAT may colocalize transiently during development or permanently with vesicular transporters for other transmitters. We have demonstrated expression of VGAT and the vesicular glutamate transporter VGLUT2 on distinct subpopulations of vesicles within a set of hippocampal nerve terminals in adult rat. Our data implicate differential release of multiple neurotransmitters from single nerve terminals in a set of GABAergic neurons. Conference: EMBO workshop: Gaba Signalling and Brain Networks , Amsterdam, Netherlands, 30 Jun - 2 Jul, 2010. Presentation Type: Poster Presentation Topic: Posters Citation: Boulland J and Chaudhry FA (2010). The vesicular GABA transporter VGAT is targeted to a distinct set of synaptic vesicles. Conference Abstract: EMBO workshop: Gaba Signalling and Brain Networks . doi: 10.3389/conf.fnins.2010.15.00005 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 23 Jun 2010; Published Online: 23 Jun 2010. * Correspondence: Farrukh A Chaudhry, University of Oslo, The Biotechnology Centre and Centre for Molecular Biology and Neuroscience, Oslo, Norway, f.a.chaudhry@biotek.uio.no Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Jean-Luc Boulland Farrukh A Chaudhry Google Jean-Luc Boulland Farrukh A Chaudhry Google Scholar Jean-Luc Boulland Farrukh A Chaudhry PubMed Jean-Luc Boulland Farrukh A Chaudhry Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.