Abstract
A comparative study was made of the sequence of the GBSS1 gene fragment in accessions of closely related species Elymus margaritae, E. komarovii, and E. transbaicalensis from Siberia. Nucleotide sequences of the gene were determined for St subgenomes in accessions of E. margaritae and E. komarovii from classical habitats. The StH-genomic constitution was confirmed, and microevolutionary relationships between species were evaluated by constructing the NJ dendrogram. It was shown that variants of the St subgenomes in accessions E. margaritae GUK-1009 and E. komarovii AUK-9803 belong to the North American ancestral line St2 of the genus Pseudoroegneria, in contrast to accessions E. margaritae AUK-0650 and E. komarovii GAR-0501. The latter belong to the Asian branch of St1, together with variants of subgenomes in the species E. gmelinii and E. pendulinus. That is, according to the differentiation levels of the St subgenome, accession E. margaritae GUK-1009 (holotype) is significantly distanted from the accession AUK-0650 (paratype), which in turn is close to accessions of E. komarovii and E. transbaicalensis from East Sayan. According to levels of differentiation of the H subgenome, the studied species did not show noticeable differences.
Highlights
Besides accessions of three mentioned species, the comparative research included clones of previously studied reference species from different regions of Asian Russia, which we assigned to two different groups according to St subgenome classification: E. kamczadalorum and E. jacutensis (St1), E. kronokensis and E. caninus (St2) (Table 1)
That variants of St subgenomes of E. margaritae GUK-1009 and E. komarovii AUK-9803 accessions belong to the North American ancestral line St2 of Pseudoroegneria genus together with reference species E. caninus and E. kronokensis, unlike the accessions of E. margaritae AUK-0650 and E. komarovii GAR-0501
The latter accessions most probably belong to Asian St1 clade with St subgenome variants of E. gmelinii and E. pendulinus species [2]
Summary
The species Elymus margaritae A.V. Agaf., Kobozeva et B. Salomon was described in collectings from the Altai Republic (holotype: Ust-Koksinsky District, Krasnaya Gora; paratype: Kosh-Agachsky District, Ukok Plateau), but initially these accessions were referred to E. komarovii (Nevski) Tzvelev [1]. Before the description of the new species E. margaritae, plants were observed in conditions of open ground and climate chamber when generations changing. The conclusion was drawn, that specimens of the supposed new species maintained natural morphology, which is significantly different from the typical E. komarovii forms. Actual questions when studying a new E. margaritae species are its origination, variability, genetic specificity and crossability with other species
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