The value of mycobacterial strains identified by the identification of 16s～23s rDNA spacer region with PCR-RFLP

Abstract

Objective To study the value in the myeobacterial strains identified by the identification of 16s~23s rDNA spacer region with PCR-RFLP. Methods The 16s~23s rDNA spacer regions in 19 species of frequent mycobacterial standard strains were amplified by PCR and the amplified products were subjected to RFLP using the restriction enzymes, Hae Ⅲ, MspⅠ, by which the differences among the amplified fragments and among the restriction fragment length polymorphism （RFLP） were analyzed. Results The results of PCR amplifica- tion showed thatl or 2 bands were always amplified in the mycobacterial strains, that the amplified fragment of slow grower mycobacteria was one between 340 and 480 bp, rapid grower mycobacteria 470 and 575 bp. 33.3% strains could be identified by the amplified products of agarose gel electrophoresis（AGE） and the results digested by the restriction enzymes showed that the macrorestriction maps of the mycobacterial strains were different. Conclusions PCR amplification of the quickly and effective tool 16s~23s rDNA spacer regions s for rapid identification of and RFLP analysis were reliable, mycobacterial strains. Key words: Mycobacteria PCR RFLP