The value of intraoperative Gram stain in revision spine surgery

Abstract

Background contextIntraoperative cultures and Gram stains are often obtained in cases of revision spine surgery even when clinical signs of infection are not present. The clinical utility and cost-effectiveness of this behavior remain unproven. PurposeThe aim was to evaluate the clinical utility and cost-effectiveness of routine intraoperative Gram stains in revision spine surgery. Study designThis was a retrospective clinical review performed at an academic center in an urban setting. Patient sampleOne hundred twenty-nine consecutive adult revision spine surgeries were performed. Outcome measuresThe outcome measures included intraoperative Gram stains. MethodsWe retrospectively reviewed the records of 594 consecutive revision spine surgeries performed by four senior surgeons between 2008 and 2013 to identify patients who had operative cultures and Gram stains performed. All revision cases including cervical, thoracic, and lumbar fusion and non-fusion, with and without instrumentation were reviewed. One hundred twenty-nine (21.7%) patients had operative cultures obtained and were included in the study. ResultsThe most common primary diagnosis code at the time of revision surgery was pseudarthrosis, which was present in 41.9% of cases (54 of 129). Infection was the primary diagnosis in 10.1% (13 of 129) of cases. Operative cultures were obtained in 129 of 595 (21.7%) cases, and 47.3% (61 of 129) were positive. Gram stains were performed in 98 of 129 (76.0%) cases and were positive in 5 of 98 (5.1%) cases. Overall, there was no correlation between revision diagnosis and whether or not a Gram stain was obtained (p=.697). Patients with a history of prior instrumentation were more likely to have a positive Gram stain (p<.0444). Intraoperative Gram staining was found to have a sensitivity of 10.9% (confidence interval [CI] 3.9%–23.6%) and specificity of 100% (CI 93.1%–100%). The positive and negative predictive values were 100% (CI 48.0%–100%) and 57.3% (CI 45.2%–66.2%), respectively. Kappa coefficient was calculated to be 0.1172 (CI 0.0194–0.2151). The cost per discrepant diagnosis (total cost/number discrepant) was $172.10. ConclusionsThis study demonstrates that while very specific for infection, the sensitivity of intraoperative Gram staining is low, and agreement between positive cultures and Gram stains is very poor. Gram staining demonstrated limited cost-effectiveness because of the low prevalence of findings that altered patient management.