Abstract
A novel seawater-based pretreatment process was developed to improve the hydrolysis yield of brown (Laminaria digitata), green (Ulva linza) and red (Porphyra umbilicalis) macroalgae. Pre-treated with 5% sulphuric acid at 121 °C, 15 minutes, L. digitata, U. linza and P. umbilicalis liberated 64.63 ± 0.30%, 69.19 ± 0.11% and 63.03 ± 0.04% sugar in seawater compared with 52.82 ± 0.16%, 45.93 ± 0.37% and 48.60 ± 0.07% in reverse-osmosis water, respectively. Low hydrolysis yields (2.6–11.7%) were observed in alkali and hydrothermal pretreatment of macroalgae, although seawater led to relatively higher yields. SEM images of hydrolyzed macroalgae showed that reverse-osmosis water caused contortions in the remaining cell walls following acid and hydrothermal pre-treatments in the L. digitata and U. linza samples. Fed-batch fermentations using concentrated green seaweed hydrolysates and seawater with marine yeast Wickerhamomyces anomalus M15 produced 48.24 ± 0.01 g/L ethanol with an overall yield of 0.329 g/g available sugars. Overall, using seawater in hydrolysis of seaweed increased sugar hydrolysis yield and subsequent bioethanol production.
Highlights
A novel seawater-based pretreatment process was developed to improve the hydrolysis yield of brown (Laminaria digitata), green (Ulva linza) and red (Porphyra umbilicalis) macroalgae
Besides the issues associated with land usage, significant quantities of fresh water are used during bioethanol production
Compared with the 1st and 2nd generation bioethanol production processes, seaweed hydrolysis yield is much lower than that achieved in the hydrolysis of starch materials and cellulosic biomass[21], indicating further improvement in seaweed hydrolysis is required
Summary
A novel seawater-based pretreatment process was developed to improve the hydrolysis yield of brown (Laminaria digitata), green (Ulva linza) and red (Porphyra umbilicalis) macroalgae. Macroalgae are a promising feedstock for the production of bioethanol, since they do not require fresh water to grow They are available in abundance, are not considered a major food source in Europe, do not occupy arable land or require fertilisers to grow[4]. The UK is a recognised centre for macroalgae biodiversity with around 644 different species inhabiting its coastal waters[5] Despite this abundance and diversity, harvested quantities are currently low (~3000 tonnes dry weight annually)[6]. Compared with the 1st and 2nd generation bioethanol production processes, seaweed hydrolysis yield is much lower than that achieved in the hydrolysis of starch materials and cellulosic biomass[21], indicating further improvement in seaweed hydrolysis is required
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have