The utilization of organic nitrogen for growth of algae: physiological aspects

Abstract

AbstractThe ability of 27 algae belonging to 11 taxonomic divisions to grow at the expense of organic nitrogen was tested in axenic culture. Experiments were carried out in buffered media: morpholine propanesulphonic acid (MOPS) was used successfully for all fresh water strains except diatoms. It was not used as a nitrogen source by any strain examined but served as a good source of sulphur for four strains. The range of substrates used was extensive and included amino acids, urea, acetamide, urate and some nucleosides. Growth rates varied widely but growth yields were generally comparable to those attained with nitrate or ammonia except for substrates containing more than one utilizable nitrogen atom. Limited experiments were carried out in the dark, and it was found that a given substrate was equally suitable for both dark heterotrophic and photolithotrophic growth. Levels of chlorophyll a were measured during growth of two algae with nitrate, glycine and urate. With nitrate, levels of specific chlorophyll a declined some ten‐fold during growth: with poor substrates supporting only slow growth, levels were more uniform but were only 10–15% of those attained during growth with good substrates. One strain grown with MOPS as sole source of sulphur produced cultures with levels of chlorophyll a about half those found in sulphate‐grown cultures. Tolerance of sodium chloride was examined in a few strains and even putatively fresh water strains were found to be appreciably tolerant.