Abstract

Objective To evaluate the accuracy of fine needle aspiration (FNA) cytology (FNAC)combined with flow cytometry (FCM) in diagnosing and classifying B-cell non-Hodgkin lymphoma (B-NHL).Methods FNA was performed on 17 patients with histopathology-diagnosed lymphoma.Some of FNA material was smeared on slides and stained by Wright' s stain,and then cell morphology was observed under microscope.The left material was prepared to cell suspensions and tested for immunophenotype with flow cytometry.According to the 2008 WHO NHL classification,the B-NHL was diagnosed and classified through FNAC combined with FCM.The results were compared with histopathology results.Results In 17 cases of B-NHL which were diagnosed by histopathology,15 cases were diagnosed by FNAC combined with FCM.Among the two negative cases,one was due to insufficient material and the other was diagnosed as reactive lymphoid tissue hyperplasia.So,the coincidence rates of diagnose and classification were 93.8 % and 93.3 %.Conclusion FNAC combined with FCM may afford sufficient evidence to diagnose and classify B-NHL,especially for those cases that are highly suspicious for lymphoma but difficult to get histopathology. Key words: Lymphoma, non-Hodgkin; Biopsy, fine-needle; Cell cytology; Flow cytometry

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call