The Utilization of Coconut Water Waste As A Growth Media of The In Vitro Potato Cutting

Hafsan Hafsan,Isna Rasdianah Aziz,Mustakim Mustakim,Masriany Masriany,Muhammad Khalifah Mustami

doi:10.24235/sc.educatia.v7i2.2636

Abstract

The limited number of potato seed caused the demand The demand to be unfulfilled especially in South Sulawesi. The use of pathogen-free seeds is absolutely necessary that can be obtained through tissue culture. This study aims to determine the effect of adding coconut water on the growth of in vitro potato plant cuttings and the most effective type of treatment. This research used Varying Concentration of the coconut water, then measure its effect on potato growth (a high number of leaves and plantlets). This study consisted of 4 treatments: TQ0 (without coconut milk), TQ1 (100 ml/l), TQ2 (150ml/l), and TQ3 (200 ml/l). The results showed that the addition of coconut water on the growth of micro cuttings of potato ( Solanum tuberosum L.) gave significant effect on the observed parameters, leaf number and plantlets weight. While TQ2 gave the best effect on the growth of in vitro micro cutting of potato.

Highlights

Potatoes are one of the horticultural commodities which needed every year in the food system in Indonesia (Timmer, 2003; Rahayu et al, 2015)
This study consisted of 4 treatments (4 levels of coconut water concentration, ie. the TQ0, TQ 1 (50 ml / l), TQ 2 (100ml / l), and TQ 3 (150 ml/ l)) and 3 replications with completely randomized design (CRD)
After the inoculation of the culture bottle mouth should be covered with plastic paper and labeled according to the concentration of the medium, and placed in tissue culture bottle rack with a culture room temperature of 21°C. 2.4 The Parameters

Summary

Introduction

Potatoes are one of the horticultural commodities which needed every year in the food system in Indonesia (Timmer, 2003; Rahayu et al, 2015). The potato development was implemented through intensification called INMAS (Intensifikasi Massal or mass intensification) and extension programs at production centers (Resosudarmo and Yamazaki, 2011). Indonesia’s potato production centers are among others located in West Java, Central Java, East Java, North Sumatra, West Sumatra, Jambi, and South Sulawesi (Fuglie et al, 2006). A majority of farmers recycle their own seeds or get them from informal sources (Muthoni et al, 2013). This limited number of good quality potato seed caused td

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