Abstract
IntroductionFallopian tube, which is normally discarded in surgical procedures, has proven to be a source of mesenchymal stem cells (MSCs) with increasing evidence. However, fallopian tube mucosa, which can be acquired via non-invasive procedures, is a previously unknown source of MSCs. In the present study, we explored the existence of MSCs in the human fallopian tube mucosa and also compared multipotent stem cells derived from fallopian tubes and fallopian tube mucosa according to their biological characteristics and therapeutic potential for treatment of autologous reproductive tract injury.MethodsCells isolated from human fallopian tubes and fallopian tube mucosa were expanded and characterised by flow cytometry. The proliferative capacity of both cell types was measured by performing colony-forming unit-fibroblast and Cell Counting Kit-8 assays. Both cell types underwent in vitro adipogenic, chondrogenic, and osteogenic differentiation. The expression of osteocyte-, adipocyte-, and chondrocyte-related genes in the differentiated cell lineages was assessed by reverse transcription-polymerase chain reaction. The secretion of growth factors and immunomodulatory cytokines by both cell types were measured by enzyme-linked immunosorbent assays.ResultsWe found that MSCs existed in the fallopian tube mucosa. The comparison between human fallopian tube MSCs (hFTMSCs) and human fallopian tube mucosa MSCs (hFMMSCs) showed that hFTMSCs had a stronger proliferative capacity and shorter duplication time than hFMMSCs. Both cell types could be differentiated into adipocytes, osteoblasts, or chondrocytes in vitro. Real-time polymerase chain reaction analysis demonstrated that hFTMSCs displayed increased expression of osteogenic-specific genes compared with hFMMSCs, but the two types of cells showed no significant increase in the mRNA expression of adipogenic-specific or chondrogenic-specific genes. hFMMSCs and hFTMSCs robustly produced a variety of growth factors and immunomodulatory cytokines.ConclusionsHuman fallopian tube mucosa is a novel source of multipotent cells. hFMMSCs demonstrated stronger proliferative capacity and superior secretion of growth factors and immunomodulatory cytokines than hFTMSCs, making the former a better source of stem cells for the treatment of autologous reproductive tract injury. Compared with fallopian tube, fallopian tube mucosa has more wide-ranging applications and can be used to carry out autologous transplantation.
Highlights
Fallopian tube, which is normally discarded in surgical procedures, has proven to be a source of mesenchymal stem cells (MSCs) with increasing evidence
The flow cytometric analyses revealed that Human fallopian tube mesenchymal stem cell (hFTMSC) and Human fallopian tube mucosa mesenchymal stem cell (hFMMSC) were positive for mesenchymal markers (CD90, CD73, and CD105) and cell adhesion molecules (CD166 and HLA-ABC) and were negative for hematopoietic lineage markers (CD34, CD45, CD14, HLA-DR, and CD19)
Our study demonstrated that hFMMSCs have a more powerful proliferative capacity than hFTMSCs. hFTMSCs displayed superiority over hFMMSCs in bone formation, but the two types of cells showed no significant increase in the mRNA expression of chondrogenic- and adipogenic-specific genes. hFMMSCs and hFTMSCs robustly produced a variety of growth factors, including basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), Granulocyte-macrophage colony-stimulating factor (GM-CSF), hepatocyte growth factor (HGF), and vascular endothelial growth factor (VEGF)
Summary
Fallopian tube, which is normally discarded in surgical procedures, has proven to be a source of mesenchymal stem cells (MSCs) with increasing evidence. Previous studies have reported the presence of mesenchymal multipotent cells in many human tissue mucosae, such as endometrium, oral mucosa, intestinal mucosa, ethmoid sinus mucosa, and olfactory mucosa; no studies have shown that multipotent stem cells are located in the fallopian tube mucosa [9,10,11,12,13,14]. We speculated that multipotent stem cells might exist in the lamina propria of the fallopian tube mucosa. We compared multipotent stem cells derived from fallopian tubes and fallopian tube mucosa according to their biological char acteristics and therapeutic potential and assessed their potential for treatment of autologous reproductive tract injury
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
