Abstract
A couple of triple and dual staining methods [high iron diamine (HID)-alcian blue (AB) pH 2.5-periodic acid-Schiff (PAS) and HID-PAS] were successfully utilized to analyze mucosubstances elaborated and released by epidermal mucous cells of four Indian freshwater fish species in light microscopy. In addition, other single and dual staining methods such as PAS, HID, AB pH 1.0, AB pH 2.5, AB pH 1.0-PAS, AB pH 2.5-PAS and HID-AB pH 2.5 were employed to further analyze the same substances and to substantiate the results obtained by the HID-AB pH 2.5-PAS and HID-PAS techniques. The comprehensive uses of pre-lectin histochemical procedures including the HID-AB pH 2.5-PAS and HID-PAS methods were unusually useful to study the cytochemical properties of mucosubstances of different mucous cell types involved in the epidermis of four Indian freshwater fish species.
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