Abstract
BackgroundSec63 is a key component of the protein translocation machinery in the mammalian endoplasmic reticulum (ER), and involved in the post-translation processing of secretory proteins. The aim of this study was to determine the expression pattern of SEC63 gene in mouse uterus during the early pregnancy.MethodsReal-time quantitative PCR and Western blot analyses were used to evaluate the alteration in levels of uterine SEC63 gene expression during the peri-implantation period in mice. Further, both in situ hybridization and immunohistochemical analyses were performed to examine the spatial localization of SEC63 gene expression in mouse uterine tissues. The presence of Sec63 protein in human uterine tissue was also detected by immunohistochemical analysis. Statistical analysis was carried out using Tukey test.ResultsUterine SEC63 gene expression was up-regulated and predominantly localized in mouse decidual cells during days 5–8 of pregnancy. More interestingly, Sec63 protein was also detected in human decidua of 10-week pregnancy, whereas was not observed in human endometrial tissues both at proliferative and secretory phases of menstrual cycle.ConclusionThe pattern of SEC63 gene expression is consistent with a possible role for SEC63 in decidualization.
Highlights
Sec63 is a key component of the protein translocation machinery in the mammalian endoplasmic reticulum (ER), and involved in the post-translation processing of secretory proteins
The pattern of SEC63 gene expression is consistent with a possible role for SEC63 in decidualization
The results showed that SEC63 mRNA was widely expressed in all of the tissues tested (Fig. 1)
Summary
Sec is a key component of the protein translocation machinery in the mammalian endoplasmic reticulum (ER), and involved in the post-translation processing of secretory proteins. Successful implantation depends on the synchronized development of a normal embryo to the blastocyst stage, and the maternal uterus from a non-receptive to a receptive state, as well as the establishment of the active interactions between maternal and embryonic tissues [3,4]. This exquisite coordination involves the regulated production of hormonal and nonhormonal molecules by embryonic and maternal tissues [5,6]. To identify novel genes that could be crucial for embryo implantation and to explore their biological roles in implantation would undoubtedly accelerate a better insight into the molecular mechanism underlying embryo implantation
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
