The using of gene-oriented SCoT markers in genotyping of the Apple genus (Malus Mill.)

Ilya Stepanov,Ilnur Balapanov,Ivan Suprun,Elena Lobodina,E Egorov,N Zaporozhets,I Ilina,E Yakimenko

doi:10.1051/bioconf/20213402005

Ilya Stepanov, Ilnur Balapanov + Show 6 more

Open Access

https://doi.org/10.1051/bioconf/20213402005

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Abstract

This paper discusses the aspects of optimization of the SCoT genotyping method for representatives of the genus Apple (Malus Mill.), including the orchard apple (Malus domestica). Special attention is paid to the methods of total DNA isolation from apple plant tissue, which is due to the sensitivity of multilocus marker systems to the quality of the nucleic acid preparation. On a sample of total DNA isolated from the leaves of the Golden Delicious cultivar, 18 SCoT markers were tested, from which 4 Malus Mill cultivars and species most promising for genotyping were selected.

Highlights

Maintaining high planting material varietal purity is one of the leading factors in the effective horticultural system management
This paper discusses the aspects of optimization of the Start Codon Targeted (SCoT) genotyping method for representatives of the genus Apple (Malus Mill.), including the orchard apple (Malus domestica)
A new type of molecular marker known as Start Codon Targeted (SCoT) targeting a short ATG start codon in plant genes was developed by a team leded Collard and Mackill

Summary

Introduction

Maintaining high planting material varietal purity is one of the leading factors in the effective horticultural system management. The control of varietal purity is most importantly updated when purchasing foreign planting material In this regard, express methods are needed to assess the varietal affiliation of the planting material at the stage of one-two-year-old seedlings. A new type of molecular marker known as Start Codon Targeted (SCoT) targeting a short ATG start codon in plant genes was developed by a team leded Collard and Mackill. This marker system has several advantages over more common marker types such as RAPD, ISSR and AFLP. These advantages include the stability of genotyping results, the presence of a larger number of amplified DNA fragments, and the reliability of the information obtained, which is due to the linked inheritance of these markers with functional plant genes

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